rnascope® h202 & protease plus reagents Search Results


90
Laboratoire Mat h2o2 3% hr-0133
H2o2 3% Hr 0133, supplied by Laboratoire Mat, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
h2o2 3% hr-0133 - by Bioz Stars, 2026-10
90/100 stars
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90
HEIDENHAIN Ltd haematoxylin destained with h202
Haematoxylin Destained With H202, supplied by HEIDENHAIN Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
haematoxylin destained with h202 - by Bioz Stars, 2026-10
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90
Merck KGaA h202
A. - D. Primary hippocampal neuronal cells were treated with 650μM, 3.25mM and 6.5mM of estetrol alone or in combination with 100nM E2 and/or 1mM P4 for 1h after induction of oxidative stress by 100μM of <t>H202</t> for 30min. A. - B. LDH activity was significantly downregulated in all the study groups compared to the H202-treated group. The LDH activity level was significantly lower in cultures treated either with 6.5mM E4 and 100nM E2 (A) or in cultures treated by any dose of E4 along with 1mM P4 than in cultures treated by E4 alone (B) as well as in cultures combinedly treated by any dose of E4 along with 1mM P4 and 100nM E2 than in cultures treated by E4 alone (A-B). C. Cell survival was significantly upregulated in cell cultures treated either by 6.5mM E4 with/without 100nM E2 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. Cells exposed to 6.5mM E4 with/without 100nM E2 had significantly higher cell survival rate than the cultures treated by 650μM E4 with/without 100nM E2. Cells combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 6.5mM E4 with/without 100nM E2. D. Cell survival was significantly upregulated in cultures treated by 6.5mM E4 with/without 1mM P4 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. The dose-dependent pattern was observed when 650μM, 3.25mM and 6.5mM E4 were used with/without 1mM P4. (C-D) Cell cultures combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 650μM, 3.25mM E4 in combination with 100nM E2 and 1mM P4. All measurements are expressed as mean±SEM.* p ≤ 0.05.
H202, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope%C2%AE+h202+%26+protease+plus+reagents/h202/pmc05085115-259-45-48
Average 90 stars, based on 1 article reviews
h202 - by Bioz Stars, 2026-10
90/100 stars
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86
Procell Inc h202
A. - D. Primary hippocampal neuronal cells were treated with 650μM, 3.25mM and 6.5mM of estetrol alone or in combination with 100nM E2 and/or 1mM P4 for 1h after induction of oxidative stress by 100μM of <t>H202</t> for 30min. A. - B. LDH activity was significantly downregulated in all the study groups compared to the H202-treated group. The LDH activity level was significantly lower in cultures treated either with 6.5mM E4 and 100nM E2 (A) or in cultures treated by any dose of E4 along with 1mM P4 than in cultures treated by E4 alone (B) as well as in cultures combinedly treated by any dose of E4 along with 1mM P4 and 100nM E2 than in cultures treated by E4 alone (A-B). C. Cell survival was significantly upregulated in cell cultures treated either by 6.5mM E4 with/without 100nM E2 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. Cells exposed to 6.5mM E4 with/without 100nM E2 had significantly higher cell survival rate than the cultures treated by 650μM E4 with/without 100nM E2. Cells combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 6.5mM E4 with/without 100nM E2. D. Cell survival was significantly upregulated in cultures treated by 6.5mM E4 with/without 1mM P4 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. The dose-dependent pattern was observed when 650μM, 3.25mM and 6.5mM E4 were used with/without 1mM P4. (C-D) Cell cultures combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 650μM, 3.25mM E4 in combination with 100nM E2 and 1mM P4. All measurements are expressed as mean±SEM.* p ≤ 0.05.
H202, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope%C2%AE+h202+%26+protease+plus+reagents/adipose+derived+human/pmc12808274-57-9-7
Average 86 stars, based on 1 article reviews
h202 - by Bioz Stars, 2026-10
86/100 stars
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90
Schmid GmbH digestion with h2s04 and h202
A. - D. Primary hippocampal neuronal cells were treated with 650μM, 3.25mM and 6.5mM of estetrol alone or in combination with 100nM E2 and/or 1mM P4 for 1h after induction of oxidative stress by 100μM of <t>H202</t> for 30min. A. - B. LDH activity was significantly downregulated in all the study groups compared to the H202-treated group. The LDH activity level was significantly lower in cultures treated either with 6.5mM E4 and 100nM E2 (A) or in cultures treated by any dose of E4 along with 1mM P4 than in cultures treated by E4 alone (B) as well as in cultures combinedly treated by any dose of E4 along with 1mM P4 and 100nM E2 than in cultures treated by E4 alone (A-B). C. Cell survival was significantly upregulated in cell cultures treated either by 6.5mM E4 with/without 100nM E2 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. Cells exposed to 6.5mM E4 with/without 100nM E2 had significantly higher cell survival rate than the cultures treated by 650μM E4 with/without 100nM E2. Cells combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 6.5mM E4 with/without 100nM E2. D. Cell survival was significantly upregulated in cultures treated by 6.5mM E4 with/without 1mM P4 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. The dose-dependent pattern was observed when 650μM, 3.25mM and 6.5mM E4 were used with/without 1mM P4. (C-D) Cell cultures combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 650μM, 3.25mM E4 in combination with 100nM E2 and 1mM P4. All measurements are expressed as mean±SEM.* p ≤ 0.05.
Digestion With H2s04 And H202, supplied by Schmid GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope%C2%AE+h202+%26+protease+plus+reagents/digestion+with+h2s04+and+h202/10__4319_slash_lo__1977__22__3__0511-25-15-16
Average 90 stars, based on 1 article reviews
digestion with h2s04 and h202 - by Bioz Stars, 2026-10
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86
Fisher Scientific additionné de h202
A. - D. Primary hippocampal neuronal cells were treated with 650μM, 3.25mM and 6.5mM of estetrol alone or in combination with 100nM E2 and/or 1mM P4 for 1h after induction of oxidative stress by 100μM of <t>H202</t> for 30min. A. - B. LDH activity was significantly downregulated in all the study groups compared to the H202-treated group. The LDH activity level was significantly lower in cultures treated either with 6.5mM E4 and 100nM E2 (A) or in cultures treated by any dose of E4 along with 1mM P4 than in cultures treated by E4 alone (B) as well as in cultures combinedly treated by any dose of E4 along with 1mM P4 and 100nM E2 than in cultures treated by E4 alone (A-B). C. Cell survival was significantly upregulated in cell cultures treated either by 6.5mM E4 with/without 100nM E2 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. Cells exposed to 6.5mM E4 with/without 100nM E2 had significantly higher cell survival rate than the cultures treated by 650μM E4 with/without 100nM E2. Cells combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 6.5mM E4 with/without 100nM E2. D. Cell survival was significantly upregulated in cultures treated by 6.5mM E4 with/without 1mM P4 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. The dose-dependent pattern was observed when 650μM, 3.25mM and 6.5mM E4 were used with/without 1mM P4. (C-D) Cell cultures combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 650μM, 3.25mM E4 in combination with 100nM E2 and 1mM P4. All measurements are expressed as mean±SEM.* p ≤ 0.05.
Additionné De H202, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope%C2%AE+h202+%26+protease+plus+reagents/additionn%C3%A9+de+h202/coutsinos_zoi__1994__etude_des_modes_d_induction_d_une_immunite_mucosale_contre_le_virus_de_l_immunodeficience_humaine_chez_la-2912-1-5
Average 86 stars, based on 1 article reviews
additionné de h202 - by Bioz Stars, 2026-10
86/100 stars
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86
Merck & Co h202
A. - D. Primary hippocampal neuronal cells were treated with 650μM, 3.25mM and 6.5mM of estetrol alone or in combination with 100nM E2 and/or 1mM P4 for 1h after induction of oxidative stress by 100μM of <t>H202</t> for 30min. A. - B. LDH activity was significantly downregulated in all the study groups compared to the H202-treated group. The LDH activity level was significantly lower in cultures treated either with 6.5mM E4 and 100nM E2 (A) or in cultures treated by any dose of E4 along with 1mM P4 than in cultures treated by E4 alone (B) as well as in cultures combinedly treated by any dose of E4 along with 1mM P4 and 100nM E2 than in cultures treated by E4 alone (A-B). C. Cell survival was significantly upregulated in cell cultures treated either by 6.5mM E4 with/without 100nM E2 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. Cells exposed to 6.5mM E4 with/without 100nM E2 had significantly higher cell survival rate than the cultures treated by 650μM E4 with/without 100nM E2. Cells combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 6.5mM E4 with/without 100nM E2. D. Cell survival was significantly upregulated in cultures treated by 6.5mM E4 with/without 1mM P4 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. The dose-dependent pattern was observed when 650μM, 3.25mM and 6.5mM E4 were used with/without 1mM P4. (C-D) Cell cultures combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 650μM, 3.25mM E4 in combination with 100nM E2 and 1mM P4. All measurements are expressed as mean±SEM.* p ≤ 0.05.
H202, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope%C2%AE+h202+%26+protease+plus+reagents/h202/10__1128_slash_mcb__00344___16-46-7-10
Average 86 stars, based on 1 article reviews
h202 - by Bioz Stars, 2026-10
86/100 stars
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90
Anwendung GmbH intrazellularen h202-entwicklung
A. - D. Primary hippocampal neuronal cells were treated with 650μM, 3.25mM and 6.5mM of estetrol alone or in combination with 100nM E2 and/or 1mM P4 for 1h after induction of oxidative stress by 100μM of <t>H202</t> for 30min. A. - B. LDH activity was significantly downregulated in all the study groups compared to the H202-treated group. The LDH activity level was significantly lower in cultures treated either with 6.5mM E4 and 100nM E2 (A) or in cultures treated by any dose of E4 along with 1mM P4 than in cultures treated by E4 alone (B) as well as in cultures combinedly treated by any dose of E4 along with 1mM P4 and 100nM E2 than in cultures treated by E4 alone (A-B). C. Cell survival was significantly upregulated in cell cultures treated either by 6.5mM E4 with/without 100nM E2 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. Cells exposed to 6.5mM E4 with/without 100nM E2 had significantly higher cell survival rate than the cultures treated by 650μM E4 with/without 100nM E2. Cells combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 6.5mM E4 with/without 100nM E2. D. Cell survival was significantly upregulated in cultures treated by 6.5mM E4 with/without 1mM P4 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. The dose-dependent pattern was observed when 650μM, 3.25mM and 6.5mM E4 were used with/without 1mM P4. (C-D) Cell cultures combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 650μM, 3.25mM E4 in combination with 100nM E2 and 1mM P4. All measurements are expressed as mean±SEM.* p ≤ 0.05.
Intrazellularen H202 Entwicklung, supplied by Anwendung GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope%C2%AE+h202+%26+protease+plus+reagents/h2so4/10__1515_slash_znb___1967___0412-51-1-0
Average 90 stars, based on 1 article reviews
intrazellularen h202-entwicklung - by Bioz Stars, 2026-10
90/100 stars
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90
AGC Inc silica support sunsphere h202
A. - D. Primary hippocampal neuronal cells were treated with 650μM, 3.25mM and 6.5mM of estetrol alone or in combination with 100nM E2 and/or 1mM P4 for 1h after induction of oxidative stress by 100μM of <t>H202</t> for 30min. A. - B. LDH activity was significantly downregulated in all the study groups compared to the H202-treated group. The LDH activity level was significantly lower in cultures treated either with 6.5mM E4 and 100nM E2 (A) or in cultures treated by any dose of E4 along with 1mM P4 than in cultures treated by E4 alone (B) as well as in cultures combinedly treated by any dose of E4 along with 1mM P4 and 100nM E2 than in cultures treated by E4 alone (A-B). C. Cell survival was significantly upregulated in cell cultures treated either by 6.5mM E4 with/without 100nM E2 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. Cells exposed to 6.5mM E4 with/without 100nM E2 had significantly higher cell survival rate than the cultures treated by 650μM E4 with/without 100nM E2. Cells combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 6.5mM E4 with/without 100nM E2. D. Cell survival was significantly upregulated in cultures treated by 6.5mM E4 with/without 1mM P4 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. The dose-dependent pattern was observed when 650μM, 3.25mM and 6.5mM E4 were used with/without 1mM P4. (C-D) Cell cultures combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 650μM, 3.25mM E4 in combination with 100nM E2 and 1mM P4. All measurements are expressed as mean±SEM.* p ≤ 0.05.
Silica Support Sunsphere H202, supplied by AGC Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope%C2%AE+h202+%26+protease+plus+reagents/silica+h+202+f/us08436112-232-54-59
Average 90 stars, based on 1 article reviews
silica support sunsphere h202 - by Bioz Stars, 2026-10
90/100 stars
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90
Carl Roth GmbH 30% h202 rotipuran® p. a., iso, stabilized
A. - D. Primary hippocampal neuronal cells were treated with 650μM, 3.25mM and 6.5mM of estetrol alone or in combination with 100nM E2 and/or 1mM P4 for 1h after induction of oxidative stress by 100μM of <t>H202</t> for 30min. A. - B. LDH activity was significantly downregulated in all the study groups compared to the H202-treated group. The LDH activity level was significantly lower in cultures treated either with 6.5mM E4 and 100nM E2 (A) or in cultures treated by any dose of E4 along with 1mM P4 than in cultures treated by E4 alone (B) as well as in cultures combinedly treated by any dose of E4 along with 1mM P4 and 100nM E2 than in cultures treated by E4 alone (A-B). C. Cell survival was significantly upregulated in cell cultures treated either by 6.5mM E4 with/without 100nM E2 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. Cells exposed to 6.5mM E4 with/without 100nM E2 had significantly higher cell survival rate than the cultures treated by 650μM E4 with/without 100nM E2. Cells combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 6.5mM E4 with/without 100nM E2. D. Cell survival was significantly upregulated in cultures treated by 6.5mM E4 with/without 1mM P4 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. The dose-dependent pattern was observed when 650μM, 3.25mM and 6.5mM E4 were used with/without 1mM P4. (C-D) Cell cultures combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 650μM, 3.25mM E4 in combination with 100nM E2 and 1mM P4. All measurements are expressed as mean±SEM.* p ≤ 0.05.
30% H202 Rotipuran® P. A., Iso, Stabilized, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope%C2%AE+h202+%26+protease+plus+reagents/30++h202+rotipuran++p++a+++iso++stabilized/pm35970349-115-9-15
Average 90 stars, based on 1 article reviews
30% h202 rotipuran® p. a., iso, stabilized - by Bioz Stars, 2026-10
90/100 stars
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90
Radikal Therapeutics indigocarmin
A. - D. Primary hippocampal neuronal cells were treated with 650μM, 3.25mM and 6.5mM of estetrol alone or in combination with 100nM E2 and/or 1mM P4 for 1h after induction of oxidative stress by 100μM of <t>H202</t> for 30min. A. - B. LDH activity was significantly downregulated in all the study groups compared to the H202-treated group. The LDH activity level was significantly lower in cultures treated either with 6.5mM E4 and 100nM E2 (A) or in cultures treated by any dose of E4 along with 1mM P4 than in cultures treated by E4 alone (B) as well as in cultures combinedly treated by any dose of E4 along with 1mM P4 and 100nM E2 than in cultures treated by E4 alone (A-B). C. Cell survival was significantly upregulated in cell cultures treated either by 6.5mM E4 with/without 100nM E2 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. Cells exposed to 6.5mM E4 with/without 100nM E2 had significantly higher cell survival rate than the cultures treated by 650μM E4 with/without 100nM E2. Cells combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 6.5mM E4 with/without 100nM E2. D. Cell survival was significantly upregulated in cultures treated by 6.5mM E4 with/without 1mM P4 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. The dose-dependent pattern was observed when 650μM, 3.25mM and 6.5mM E4 were used with/without 1mM P4. (C-D) Cell cultures combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 650μM, 3.25mM E4 in combination with 100nM E2 and 1mM P4. All measurements are expressed as mean±SEM.* p ≤ 0.05.
Indigocarmin, supplied by Radikal Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope%C2%AE+h202+%26+protease+plus+reagents/indigocarmin/10__1515_slash_znb___1966___0220-32-0-15
Average 90 stars, based on 1 article reviews
indigocarmin - by Bioz Stars, 2026-10
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90
Becton Dickinson h202-141
FACS antibodies
H202 141, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope%C2%AE+h202+%26+protease+plus+reagents/substrate+solution+tetramethyl+benzidine+%E2%81%84+h202/pmc08932531-15-2-4
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Image Search Results


A. - D. Primary hippocampal neuronal cells were treated with 650μM, 3.25mM and 6.5mM of estetrol alone or in combination with 100nM E2 and/or 1mM P4 for 1h after induction of oxidative stress by 100μM of H202 for 30min. A. - B. LDH activity was significantly downregulated in all the study groups compared to the H202-treated group. The LDH activity level was significantly lower in cultures treated either with 6.5mM E4 and 100nM E2 (A) or in cultures treated by any dose of E4 along with 1mM P4 than in cultures treated by E4 alone (B) as well as in cultures combinedly treated by any dose of E4 along with 1mM P4 and 100nM E2 than in cultures treated by E4 alone (A-B). C. Cell survival was significantly upregulated in cell cultures treated either by 6.5mM E4 with/without 100nM E2 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. Cells exposed to 6.5mM E4 with/without 100nM E2 had significantly higher cell survival rate than the cultures treated by 650μM E4 with/without 100nM E2. Cells combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 6.5mM E4 with/without 100nM E2. D. Cell survival was significantly upregulated in cultures treated by 6.5mM E4 with/without 1mM P4 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. The dose-dependent pattern was observed when 650μM, 3.25mM and 6.5mM E4 were used with/without 1mM P4. (C-D) Cell cultures combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 650μM, 3.25mM E4 in combination with 100nM E2 and 1mM P4. All measurements are expressed as mean±SEM.* p ≤ 0.05.

Journal: Oncotarget

Article Title: Use of estetrol with other steroids for attenuation of neonatal hypoxic-Ischemic brain injury: to combine or not to combine?

doi: 10.18632/oncotarget.9591

Figure Lengend Snippet: A. - D. Primary hippocampal neuronal cells were treated with 650μM, 3.25mM and 6.5mM of estetrol alone or in combination with 100nM E2 and/or 1mM P4 for 1h after induction of oxidative stress by 100μM of H202 for 30min. A. - B. LDH activity was significantly downregulated in all the study groups compared to the H202-treated group. The LDH activity level was significantly lower in cultures treated either with 6.5mM E4 and 100nM E2 (A) or in cultures treated by any dose of E4 along with 1mM P4 than in cultures treated by E4 alone (B) as well as in cultures combinedly treated by any dose of E4 along with 1mM P4 and 100nM E2 than in cultures treated by E4 alone (A-B). C. Cell survival was significantly upregulated in cell cultures treated either by 6.5mM E4 with/without 100nM E2 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. Cells exposed to 6.5mM E4 with/without 100nM E2 had significantly higher cell survival rate than the cultures treated by 650μM E4 with/without 100nM E2. Cells combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 6.5mM E4 with/without 100nM E2. D. Cell survival was significantly upregulated in cultures treated by 6.5mM E4 with/without 1mM P4 or by 3.25mM, 6.5mM E4 with 100nM E2 and 1mM P4 than in H202-treated cultures. The dose-dependent pattern was observed when 650μM, 3.25mM and 6.5mM E4 were used with/without 1mM P4. (C-D) Cell cultures combinedly treated by 6.5mM E4 with 100nM E2 and 1mM P4 had significantly higher survival level than the cells treated by 650μM, 3.25mM E4 in combination with 100nM E2 and 1mM P4. All measurements are expressed as mean±SEM.* p ≤ 0.05.

Article Snippet: To define the concentrations of E2 (Sigma-Aldrich, St. Louis, MI, USA) and P4 (Sigma-Aldrich, St. Louis, MI, USA) with antioxidative and neuroprotective properties on primary hippocampal cell cultures prepared from newborn rat pups, at day 7 after plating cells, cultures were treated with 100μM of H202 for 30min (Merck KGaA, Darmstadt, Germany) and then with different concentrations of E2 or P4 from 1nM to 1mM for 1h.

Techniques: Activity Assay

FACS antibodies

Journal: The Journal of Experimental Medicine

Article Title: Suppressive effects of the obese tumor microenvironment on CD8 T cell infiltration and effector function

doi: 10.1084/jem.20210042

Figure Lengend Snippet: FACS antibodies

Article Snippet: CD98-PE , H202-141 , BD Biosciences.

Techniques: